Cryo-electron microscopy for integrative structural biochemistry

About this programme

The course covers all typical steps of Single Particle Cryogenic Electron Microscopy (Cryo-M), from specimen preparation to atomic model building. Requirements of the sample for cryo-EM, major and most up-to-date software for performing single particle cryo-EM, as well as how Cryo-EM compares with and can be combined with other structural biology techniques are included. The course will also introduce other applications of cryogenic electron microscopy, such as cryo-electron tomography and electron diffraction of nano protein/compound crystals (microED). - design a single particle cryo-EM project and identify possible pitfalls in the process - perform a Cryo-EM experiment, from specimen preparation to model building of a target protein - Show ability to analyse dynamic systems and use a combination of prediction methods, including AlphaFold, and manual model building. Teaching consist of lectures, group instruction, seminars, exercises and laboratory sessions. Module 1 takes place through written exams, written reports and oral presentations. Course Coordinator Marta Carroni, email: marta.carroni@dbb.su.se Chemistry Section & Student Affairs Office: chemistry@su.se To follow your passion. To be close to international career opportunities. To explore city and countryside. In Stockholm you can really get the best of both worlds. Here we explain all the different steps you need to go through when applying to a course or programme. Stockholm University arranges regular webinars and participates in educational fairs and events virtually and around the world to meet stud